1. Remove the microscope from the cabinet by grsping the handle in the arm of the scope with one hand and placing the other hand below the base. Be careful not to hit the top of the cabinet with the oculars!!!
2. Always start with the low power objective (4x). It allows for a larger field of view and has a longer working distance. Use the coarse focus knob to bring your subject into rough focus and then into crisp focus using the fine focus knob.
3. Adjust for the distance between your eyes by changing the position of the two oculars on the binocular head by gently pulling them apart or pushing them together. Other students use this scope so you will need to make this adjustment every time you use the scope!
4. Adjust for the difference between the acuity of your eyes. Close your left eye and bring the subject into sharp focus for the right eye using the fine focus knob. Now close your right eye and bring the subject into sharp focus for your lrft eye using only the focus adjustment ring on the left ocular. This will take two hands. Other students use this scope so you will need to make this adjustment every time you use the scope!
5. Our objectives are parfocal. When your subject is in focus at 40x, the other two will be positioned to be roughly in focus. When switching to the 10x or 40x objective you need only use the fine focus knob to bring your subject into crisp focus.
6. Adjust the intensity of the brightness of the field using the voltage control dial not the iris diaphragm of the condenser.
7. Adjust the field iris at the magnification you will use most during that particular lab. - link to outline of this procedure.
8. When you are through, reposition the 4x objective into place, wrap the cord around the base and carefully return to the cabinet.