Through Focusing

Three views of an Elodea leaf as the objective is progressively lowered towards the subject

#1. Face view of chloroplasts - focus on the sheet of cytoplasm directly below the cell membrane.

#2. The focus is in the area of the vacuole. Note the choroplasts seen in edge-view and the nucleus encroaching into the area of the vacuole.

#3. The focus is below the top layer of cells reveling the outlines of cells making up a second layer.

Link to a movie